IB47301 Mini Total Tissue RNA Kit for 50 Preparations

The total RNA mini kit (tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required. The quality of the total RNA mini kit (tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis. Caution: RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Yield : 5-30 microgram. Binding capacity: up to 60 microgram. Operation time: 30 minutes. Applications: RT-PCR, real-time RT-PCR, northern blotting, primer extension, RNase protection assays, mRNA selection, cDNA synthesis.

• Kit contains 50 Mini RB columns, (50) filter columns, (100) 2mL collection tubes, (50) micropestle, 30mL RB buffer, 30mL W1 buffer, 12.5mL wash buffer (50mL add ethanol), 6mL RNASE-free water
• For research use only, total RNA minipreps from tissues, cultured cells and blood samples
• Sample size: up to 25mg of tissue, up to 25mg of paraffin-embedded tissue
• Spin column format
• Operation: centrifuge/vacuum manifold